Designing and Fabrication of a Biocomposite for Skin Tissue Engineering
H. MIRZADEH*1,2, H. MAHDAVIIH. MORAVEJ FARSHI3
, M. MAHMODI RAD3, P. TOSI3
1-Polymeric Biomaterials Department, Iran Polymer and Petrochemical Institute,
Tehran, Iran
2-Polymer Engineering Department Amirkabir University of Technology
3-Skin Research Center of Shahid Beheshty Medical University (SBMU), Tehran, Iran
Email:mirzadeh@aut.ac.ir
The aim of this study was to develop a quartet composite film namely IPPISkin comprising chitosan (Cs), glucose-amino-glycans (GAGs), collagen (Col)/ gelatin and polyvinylalcohol (PVA) as dermal substitute for treatment of dermal burns or injuries.
In the previous work, the physical, chemical and mechanical properties of the membrane were characterized using different techniques such as scanning electron microscopy (SEM), Fourier transform infrared (FTIR), differential scanning calorimetry (DSC) and dynamic mechanical thermal analysis (DMTA).Also the cytotoxicity and in vitro culture studies were performed using L929fibroblast cell line. The blend membrane was evaluated in vivo, using guinea pigas a model for investigating the behaviour of the membrane on the healing ofthe full wound thickness.
Recently, in a collaborative research work between IPPI and SBMU, by permission of the National Ethical Committee, several treatment tests were assessed using IPPISkin with or without cultured cell, on the voluntary patients who suffered from either burn injuries, or diseases such as Leishmaniasis or other accidents. IPPISkin clinical performance has shown to alleviate painful autografting and prolonged treatment regimens and has a high capability for wound healing management and could be considered as suitable candidate for dermal burn healing or cutaneous wounds.
